| Instructors: |
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| Lectures: |
2:30-3:45pm, Tuesdays and Fridays 608 Wood Basic Sciences (library) |
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| Text: |
Principles of Protein X-Ray Crystallography (2nd edition) by Jan Drenth |
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| Course web site: | http://biophysics.med.jhmi.edu/crystalcourse/ | ||||
| Links to crystallography teaching web sites | |||||
| Syllabus: | Dates: |
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Overview of protein crystallography; x-rays and detectors Crystals and crystal growth, indexing of lattice planes, vector and matrix review |
April 2 and 5 |
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Crystal symmetry, space groups; review of matrices and complex numbers X-ray scattering by atoms and unit cells; review of Fourier transforms |
April 9 and 12 |
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Scattering by crystals, convolution theorem Bragg’s Law, Laue conditions, Ewald construction |
April 16 and 19 |
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Isomorphous replacement The Patterson function |
April 23 and 26 |
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Difference electron density maps: 2Fo-Fc, Fo-Fc, omit maps Phase improvement: density modification, non-crystallographic symmetry |
April 30 and May 3 |
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Anomalous scattering and MAD phasing, Molecular replacement |
May 7 and 10 |
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Refinement, model accuracy. Take home exam handed out Laue method |
May 14 and 17 |
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Final lecture Final take home exam due |
May 21 |