The Eukaryotic Tissue Culture Facility at Johns Hopkins was established to aid laboratories wishing to carry out experiments that require eukaryotic cells. Services provided include advice on eukaryotic expression methods, transfection and selection of cell lines, production of baculovirus and baculovirus-infected cells, and scaling up of cell culture volumes. In particular, support for expression of proteins in both insect cells (Sf9, Hi5) and common eukaryotic cell lines (HEK293, CHO) is provided along with the capacity to produce up to 20 liters of cell suspension or conditioned medium.
The most common inputs to the facility are expression plasmids or cell lines, and the most common outputs are cell paste, conditioned medium, or high-titer baculovirus. These services can be provided by the facility, or personnel from your lab can be trained and checked out to use facility resources. Although we can provide advice, the facility is not equipped for protein purification. The pricing philosophy of the facility is one of cost recovery, and prices are determined based on the amount of supplies (plasticware, medium, etc.) and facility personnel time required. Prior to carrying out any work a cost estimate will be provided.
Please contact, the Facility Manager, Dr. Yana Li, for more information about available services and costs.
Sandra B. Gabelli, Ph.D.
James M. Berger, Ph.D.
How to start using the facility
The iLab Solutions Core Facility Management software (iLab) allows JHU researchers reserve time on equipment or submit service requests to Eukaryotic Tissue Culture Facilities. It also provides JHMI with a centralized billing system for core services. Information to assist new and existing users with accessing and navigating the system can be found on iLab ETCF.
To register for an account:
1. Click CORE at JHMI to login and register.
2. Login using your JHMI credentials.
3. Upon successful authentication, you will be prompted to select your PI/lab from the pull-down list and provide your phone number.
4. Submit the completed registration form. Submission will notify your PI of your lab membership request.
5. You will be sent an email with basic instructions on using the system at this time. However, you will not be able to place orders with your new account until your PI has approved your lab membership request.
Facility Equipment and Consumables
The facility has two locations; one at Biophysics 306 and another one at Hunterian 710. For use of the facility equipment listed below, please go to iCalendar [put the site when available] and prices tab for more information.
• Biological safety cabinets with pipette aids and vacuum aspirators
• Inverted microscopes to observe cell morphology and count cells
• CO2 incubators to culture mammalian cells, plant cells and insect cells
• 37° C water bath
• Sorval RegentRT centrifuge with swing buckets for 15ml, 50ml and 200 ml tubes
• 4° C refrigerator with freezer to store immediately needed media and reagents
• -20° C chest freezers
• -80° C ultralow freezer
• Liquid Nitrogen storage tank Glass hemocytometer for counting cell numbers
• Freezing box (VWR brand) for 1-2 ml cryogenic tube, 18 spaces
• Basic consumables such as tips, disposable pipettes, paper towels, 70% ethanol, autoclave bags/tapes
Links and Resources
ETCF Grant Resource Document
The Eukaryotic Tissue Culture Facility core provides baculovirus titer measurements, growing of cultured mammalian and insect cells, and cell storage services. In addition, the Facility advice users in cell culture techniques and provides sterile hood facilities for users. Major equipment includes biosafety cabinets; a microscope; four CO2 incubators and three incubators for insect cell lines; cold storage facilities consisting of a liquid nitrogen freezer, cryogenic storage Dewars, ultra low freezer, X 152 4° C freezers and refrigerators. The core is centrally located for the JHMI basic sciences in two rooms in the Hunterian and the Biophysics buildings with easy access for most researchers in the School of Medicine