January 11, 2018 @ 3:30 pm – 6:00 pm
Mariusz Matyszewski from the Sohn lab will present hisresearch titled “CryoEM Structure of the AIM2 filament“
Absent-In-Melanoma 2 (AIM2) is a protein involved in the humaninnate immune system. Its function is to sense foreign dsDNA, upon which it canfilament on the DNA and interact with Apoptosis-associated Speck-like proteincontaining a CARD (ASC) to start inflammasome formation. Inflammasomes arelarge, supramolecular structures used to initiate inflammatory responsesthrough interleukin activation by Caspase-1. Unlike many signaling networks,AIM2-ASC inflammasome transduces the signal through structure rather enzymaticcatalysis, in which AIM2 filaments cause ASC to filament, which then allowsCaspase-1 to dimerize and activate. To better understand the mechanism andspecificity of signal transduction between AIM2 and ASC filaments, he has beenstudying AIM2 filaments through CryoEM. In this talk, he will talk about hisefforts to optimize AIM2 for CryoEM, new insights the structure can provide,and some preliminary results about AIM2-ASC filament specificity.