Congratulations to PREP postbac, Aitana Azurmendi, who is being recognized with the inaugural post-baccalaureate Young Investigator’s Award!
Roger Zou received the Michael A. Shanoff Award from the Johns Hopkins Medicine 2021 Young Investigator’s Day Program.
Meeting ID: 940 5320 8314
Passcode: (see announcement email)
11-11:10 Meera Joshi /Woodson
11:15-11:25 Sam Canner/Sohn
11:30-11:40 Martin Yepes /Xiao
11:45-11:55 Anna Andrick /Hilser
12:10-12:20 Robbin Jang /Ha
12:25-12:35 Edgar Manriquez-Sandoval/Gray
12:40-12:50 Andrea Ori/Barrick
12:55-1:05 Qilan Wei/Ha
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Meeting ID: 975 3755 6895
“Dynamics: a palantír for enzyme and protein function”
Aswani Kancherla, Postdoctoral Fellow, Frueh Lab
Kenneth Marincin, PMB Graduate Program, Dominique Frueh, Advisor
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Examining the conformational dynamics of Na+/I– symporter (NIS) using enhanced sampling methods
Mayukh Chakrabarti (Amzel lab)
The Na+/I– symporter (NIS), a transmembrane protein found in the thyroid gland and other tissues, carries out the uptake of iodide, a required constituent of thyroid hormones T3 and T4. Physiologically, NIS transports iodide to the cytoplasm of thyroid cells with a stoichiometry of 2 Na+: 1 I–, driven by the sodium concentration gradient maintained by the Na+/K+ ATPase. Our lab, together with Dr. Carrasco’s laboratory (Vanderbilt University), developed homology models of inwardly and outwardly open NIS, based on several recently determined structures of bacterial Na+-dependent transporters. The current view of NIS transport involves four principal states that can be characterized in a thermodynamic cycle: outwardly open with no ligands bound, outwardly open with all ligands bound, inwardly open with all ligands bound, and inwardly open with no ligands bound. However, the metastable intermediate states of NIS and their populations in these thermodynamic transitions have thus far not been elucidated. We employed molecular dynamics (MD) simulations, using an enhanced sampling technique, to characterize the transition of NIS between its inwardly and outwardly open states in the absence and presence of ions, and obtain insight into the populations and free energy differences of the states along this transition pathway. This information could be used to inform testable experimental assays for further validation.